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Assessment of investment in heating house
Zdražil, Pavel ; Štelcl, Otakar (referee) ; Baláš, Marek (advisor)
The bachelor´s thesis deals with economic evaluation of investment in insulation and heating and resulting operation of boilers in a family house. The calculation of thermal design power, which is needed for heating, was made according to norm ČSN EN 12831 for the house in actual state without insulation. Further were suggested two options of insulations and modernization of windows, for reduction of heat loss. Then was calculated the thermal design power for the two new options, again according to norm ČSN EN 12831. Next were presented possibilities of heat sources. For the specified family house was chosen a gas boiler in classical and condensing implementation for all three variants of insulation. These all options have made six variants which were compared together and evaluated. In the conclusion were excluded four variants economically disadvantageous and was chosen one of the final two variants as the best suggestion for the specified family house.
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Design of HV disconnector
Hrubý, Radek ; Valenta, Jiří (referee) ; Vávra, Zdeněk (advisor)
The text focuses on the design of high-voltage disconnector. It made a brief theoretical analysis of construction equipment directives. Proposal follows the insulation system apparatus according to empirical formulas. Hereafter, we discuss the design of current track isolators. For the flow path are calculated electrodynamic forces acting. Warming current path is simulated by a computer program ANSYS, this simulation includes 3D device model and determine the material and thermal constants. Finally it is shown calculating the required torque on the shaft of the disconnector. This calculation is simplified. The output is a 3D sketch of the device model used to simulate warming.
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Hormones in waste waters
Petrušová, Pavlína ; Vávrová, Milada (referee) ; Čáslavský, Josef (advisor)
This bachelor thesis gives the overview of waste water contaminants of steroid hormones type. A short description of these compounds and of their physical-chemical properties is given and sources of these substances in the environment are characterized, as well as their impact on organisms The fate of these compounds and methods used for their isolation from environmental compartments methods of their final analysis are characterized.
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Adjustment of the Insulation of the Bus Rear Part
Pešina, Jan ; Kubík, Roman (referee) ; Kalivoda, Milan (advisor)
Master thesis is focused on proposal of improved manufacturing process of assembly named "sandwich" produced in Iveco bus company. There is solved new fixation of assembly bench, replacement of currently used glues and material of ledge as well. In the first part there is company introduction Iveco Bus. Second part consist of construction analysis and description of present manufacturing process. Further there is introduction, solving out of thesis topics and proposal of new manufacturing process is written. At the end of thesis are attached comparison between present and proposed manufacturing process.
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Multipurpose Rekreational Facility
Holá, Ludmila ; Závorková, Kateřina (referee) ; Králová, Zuzana (advisor)
This master thesis deals with the proposal of a multifunctional recreational facility. The complex of buildings consists of a guesthouse and stables. The guesthouse is a single-storey building with a residential attic. It also includes a restaurant with a mini-brewery and a shop selling horse riding products. The guesthouse is designed to accommodate up to 41 people. The first floor is barrier-free. The main entrance of the guesthouse and the shop faces southwest. The restaurant is accessible from the southeast. There are paved guest parking areas around the buildings. The building of stables with its accompanying facilities is a single-storey which is designed to stable 17 horses. The main entrance of the stables faces southeast and the building is also accessible from the northeast and the northwest. Both buildings have a gabled roof which consists of supporting rafters. The buildings are as designed a timber-frame construction with a supporting frame made of timber pillars which is coated with the FERMACELL boards.
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Preparation and purification of plasmid DNAs
Němeček, Milan ; Španová, Alena (referee) ; Rittich, Bohuslav (advisor)
With the development of therapeutic methods in medicine, like are DNA vaccines and gene therapy increases demand for new processes for the isolation and purification highly pure plasmid DNA. Most often used methods of purification plasmid DNA are chromatographic methods. In experimental part of this thesis was performed isolation of plasmid pUC-19 DNA plasmid via alkalyne lysis. And purification of plasmid was performed by liquid chromatography and agarose gel electrophoresis.
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Cryogenic Technique
Zrůst, Michael ; Milčák, Pavel (referee) ; Kracík, Petr (advisor)
Bachelor thesis deals with technology of cryogenic temperatures, liquefying gases in this temperature interval, effects of these temperatures on materials and insulations. In the introduction, brief history of development of this field. Next part is about gases which get liquefied in cryogenic temperatures and their applications. The largest chapter is chapter about liquefying in which principle of coolers, heat exchangers and liquefiers is described. Next, means of cooling by expansion, cooling cycles used to reach desired temperatures and ways of separating gas mixtures, are specified. In the last chapter insulation used to maintain cryogenic temperatures inside space is discussed.
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Comparison of different types of magnetic carriers for DNA microisolation from foods
Koplík, Jerguš ; Lunerová,, Jana (referee) ; Kovařík, Aleš (advisor)
Micro-isolation of PCR-ready from fresh and dried legumes seeds and food products containing legumes (hummus) was tested. For optimization process magnetic microparticles PGMAox was used. Optimum weight plants and food material was 200 mg. For isolation of DNA from fresh legumes, mixture containing 500 L of CTAB extraction buffer, 1 L -mercaptoethanol and 500 L chloroform-octanol was used. For isolation of DNA from dried legumes seeds and food products volume of CTAB extraction buffer was increased to 1 000 L. To achieve higher purity of DNA some prepared homogenates was precipitate by isopropylalcohol. The optimized process of DNA isolation was used to prepare a homogenate from food products which DNA was isolated by different types of magnetic carriers. For comparison, non-porous magnetic carriers poly(glycidyl methacrylate) PGMAox, poly(2-hydroxyethyl methacrylate-co-glycidyl methacrylate) P(HEMA-co-GMA)ox covered by carboxyl groups and porous fully crosslinked microparticles poly(styrene-co-divinylbenzene) HPS-B-M-22-NH2, magnetic porous glass MPG and nanoparticles of iron oxides covered by poly(L-lysine) PLL were used. In average the highest concentration and the best purity of DNA was isolated by magnetic carriers P(HEMA-co-GMA)ox a PGMAox.
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